Cost-Effective Endo-Mannanase from Bacillus Sp. Cfr1601 and its Application in Generation of Oligosaccharides from Guar Gum and as Detergent Additive

The indigenous bacteria Bacillus sp. CFR1601 produced significant levels of endomannanase
when grown on agro-wastes, namely, green gram husk and sunflower oil cake (25.6
IU=mL), used as sole carbon and nitrogen sources, respectively. Under immobilized cell system,
synthetic supports (polyurethane foam, scotch brite, polyester; up to 33.2 IU=mL) were found
marginally superior as compared to natural supports (cotton and silk; up to 28.2 IU=mL) for
endo-mannanase production. Cooperative interactions between L-lysine HCl (0.3% w=v), Tween
60 (0.3% v=v), and sunflower oil cake (3.0% w=v) in central composite design response surface
methodology ameliorated (1.61-fold) endo-mannanase titers to 48.0 IU=mL. Partially purified
endo-mannanase was tested for its ability to produce oligosaccharides from guar gum. These oligosaccharides
were tested in vitro for their ability to promote growth of Lactobacillus plantarum
MTCC 5422 and Lactobacillus salivarius CHS 1E. Results indicated that low-molecular-weight
degraded products from guar gum were (1) able to support the growth of tested strains [increased
O.D600nm up to 2.3-fold and decrease in pH (<6.3) due to production of short chain fatty acid
(SCFA)] when used as sole carbon source; and (2) after purification and analysis by electron spray
ionization–mass spectrometry (ESI-MS) were found to be composed of mainly disaccharide and
tetrasaccharide. The compatibility of endo-mannanase with various detergents together with wash
performance test confirmed its potential applicability for laundry industry.