Efect of biotic and abiotic elicitors on isofavone biosynthesis during seed development and in suspension cultures of soybean (Glycine max L.).

Akitha Devi, M. K. and Gyanendra, Kumar and Giridhar, P. (2020) Efect of biotic and abiotic elicitors on isofavone biosynthesis during seed development and in suspension cultures of soybean (Glycine max L.). 3 Biotech, 10. p. 98.

PDF 3 Biotech (2020) 1098.pdf - Published Version Restricted to Registered users only Download (2MB) | Request a copy

Abstract

The present investigation aimed to look at the efects of biotic and abiotic elicitors during Soybean seed development and cell suspension culture in isofavones accumulation. The expression levels of four major genes viz., CHS7, CHS8, IFS2, and IFS1 involved on isofavones biosynthesis during seed developmental stages from R5L–R7 was seen in both MAUS-2 and JS-335 Soybean varieties. The R7 stage showed 1.24-fold upregulation of IFS1transcript level and considered as the control for Soybean seed development. Both varieties during R6−R8 stages responded diferently to the foliar application of 10 µM SA, 10 µM MJ and 0.1% Aspergillus niger. The IFS2 transcripts were upregulated by SA at the R7 stage with 5.21- and 4.68-fold in JS-335 and MAUS-2, respectively. IFS1 expression was signifcantly increased by A. niger treatment at R7 stage with 3.98- and 3.21-fold in MAUS-2 and JS-335, respectively. The expression of CHS7 and CHS8 by 10 μM SA at R7 level revealed maximum up-regulation of 0.51- and 1.01-fold in MAUS-2; 0.37- and 0.82-fold in JS-335, respectively. In the soybean callus suspension culture, biosynthetic genes were used to validate the efects of elicitor on isofavones. Both biotic and abiotic treatments contribute to the upregulation of IFS1 and IFS2 expression, that in turn, leads to the accumulation of isofavone in seed development as well as in suspension cultures. These data further suggested that the IFS2 is the key gene responsible for the isofavone accumulation during elicitor treatment.

Item Type:	Article
Uncontrolled Keywords:	Augmentation · Callus suspension · Elicitation · Isofavones · qPCR
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 04 Biosynthesis 600 Technology > 08 Food technology > 22 Legumes-Pulses > 05 Soya bean
Divisions:	Plant Cell Biotechnology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	04 Dec 2020 05:36
Last Modified:	04 Dec 2020 05:36
URI:	http://ir.cftri.res.in/id/eprint/14684

Actions (login required)

View Item