Comparative assessment of amino acids composition in two types of marine fish silage.

Fish silage is a brown liquefied product achieved by the action of enzymes when finely grounded
whole/parts of either single or mixed fish types are subjected to acidification. This study made
a comparative assessment of biochemical and nutritive properties, especially the amino acid
composition in supernatant phase of formic acid silages prepared from two fish types, Indian
mackerel (Rastrelliger kanagurta) and false travely (Lactarius lactarius) representing fat fish (FF, fat
content > 5%) and lean fish (LF, fat content < 5%), respectively during 35 days of fermentation (DoF).
Significantly higher content of total amino acid (TAA) and free amino acids (FAA) were recorded in
FFS (TAA, 41.2 ± 0.03 mg/g; FAA, 31.3 ± 0.003 mg/g) compared to LFS (TAA, 35.8 ± 0.07 mg/g; FAA,
18.26 ± 0.003 mg/g; FAA, 31.3 ± 0.003 mg/g) (p < 0.05). At the end of 35 DoF, the concentrations of
amino acids such as asparagine, histidine, isoleucine, valine, cysteine, serine, lysine and arginine
were significantly higher in FFS as compared to LFS. The relative amino acid composition of FFS and
LFS varied in accordance with DoF and the relationship was found to be highly significant (ANOVA,
p < 0.00001). High concentrations of l-amino acids such as leucine, glutamic acid and arginine were
recorded in both FFS and LFS. In conclusion, the analysis suggested that a fermentation period of
25–30 days showed a significant effect on the composition of amino acids in both types of ensilage
compared to other fermentation periods (p < 0.05). Considering the role of amino acids in enhancing
the plant growth and proliferation, the findings of the present study are quite useful.