Novel adsorption approach for the enrichment of R-Phycoerythrin from marine macroalga Gelidium pusillum.

Rochak, M. and Richa, Sharma and Raghavarao, K. S. M. S. (2022) Novel adsorption approach for the enrichment of R-Phycoerythrin from marine macroalga Gelidium pusillum. Algal Research, 62. p. 102605.

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Abstract

R-Phycoerythrin is a fluorescent and commercially valuable pigment protein present in algae. The extraction, purification and concentration of such biomolecules is a challenge for processing industries. In the present work, enrichment of R-Phycoerythrin was attempted by selective adsorption of other contaminant biomolecules from extract of algal biomass. Different adsorbents (zeolite, molecular sieve, ammonium Y zeolite, silver-exchanged zeolite, mesoporous carbon) and nanoparticles (zinc oxide, graphene oxide, gold and silver) were explored for this purpose. Process conditions such as buffer pH (5, 6, 7 and 8), ionic strength (0.1, 0.35, 0.6, 0.85 and 1.1) and adsorbent levels (w/w) were optimized. Optimization resulted in enrichment of pre-treated R-Phycoerythrin by 3.31 fold with 73.36% yield using zeolite and 2.10 fold with 98.91% yield using gold nanoparticles. This work demonstrates the efficacy of adsorbents and nanoparticles as possible alternatives for enrichment and purification of R-Phycoerythrin with appreciable yield.

Item Type:	Article
Uncontrolled Keywords:	Enrichment Downstream processing Nanoparticles Phycobiliproteins Purification Seaweed
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 01 Algae 600 Technology > 08 Food technology > 16 Nutritive value > 03 Proteins
Divisions:	Food Engineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	09 May 2022 08:56
Last Modified:	09 May 2022 08:56
URI:	http://ir.cftri.res.in/id/eprint/15234

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