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Preparation, purification, and identification of novel antioxidant peptides from red‑bellied pacu (Piaractus brachypomus) fish meat protein hydrolysate.
Aliaa, M. A. Hashem and Anushma, Venmarath and Tanaji, G. Kudre (2023) Preparation, purification, and identification of novel antioxidant peptides from red‑bellied pacu (Piaractus brachypomus) fish meat protein hydrolysate. Food Science and Biotechnology. ISSN 1226-7708
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Abstract
The current study investigates the preparation, purifcation, and identifcation of novel antioxidant peptides from Piaractus brachypomus fsh (RBPF) meat. Antioxidant peptides from RBPF meat protein hydrolysate (RPMPH) were fractionated by ultrafltration (3 kDa MWCO membrane). RPMPH-IF (MW<3 kDa) fraction displayed signifcantly higher antioxidant activities (P<0.05) (DPPH, ABTS, FRAP, and Fe2+chelating activity). RPMPH-IF was purifed by Sephadex G-25 gel fltra�tion chromatography, and the RPMPH-1 fraction exhibited signifcantly higher antioxidant activities (P<0.05). Subsequently, the RPMPH-1 fraction was purifed by reversed-phase high-performance liquid chromatography. RPH-8 showed the highest antioxidant activities. The sequence of peptides of the RPH-8 fraction was later identifed by LC-MS/MS and MASCOT software. RPH-8 fraction showed the two peptides with MW of 1105.52 Da and 748.25 Da, and the sequence of peptides was identifed as His–Asn–Leu–Gly–Leu–Leu–His–Gly–Asp–Met and Asp–Ala–Pro–Ser–Met–Asn–Asp, respectively. Thus, RPMPH or purifed antioxidant peptides produced by probiotic Bacillus strain could be a bio-functional ingredient in food and nutraceutical applications.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Piaractus brachypomus fsh meat · Fermentative hydrolysis · Antioxidant activity · Peptide purification · Peptide identifcation |
| Subjects: | 600 Technology > 08 Food technology > 16 Nutritive value > 03 Proteins 600 Technology > 08 Food technology > 28 Meat, Fish & Poultry > Fish |
| Divisions: | Meat Fish and Poultry Technology |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 25 May 2023 04:13 |
| Last Modified: | 25 May 2023 04:13 |
| URI: | http://ir.cftri.res.in/id/eprint/16463 |
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