Transcriptomic Profiling of Resistant and Susceptible Rice Cultivar Blast Resistance Genes During Magnaporthe oryzae Infection.

Chandrakanth, R. and Sunil, L. and Devaki, N. S. (2023) Transcriptomic Profiling of Resistant and Susceptible Rice Cultivar Blast Resistance Genes During Magnaporthe oryzae Infection. Plant Molecular Biology Reporter. pp. 1-11.

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Abstract

Rice is an important staple food crop and a primary energy source for more than half of the world’s population. One of the deadly fungal diseases of rice produced by Magnaporthe oryzae is known as “blast disease.” It has consistently threatened the world’s food supply. This study aimed to dissect the complex blast resistance mechanism of the rice plant against blast pathogens. To understand the molecular mechanisms of blast resistance, we analyzed transcriptome changes in rice cultivars. Using transcriptome data obtained from an RNA-seq analysis of two samples comprising resistant (BR2655) and susceptible (HR12) genotypes under control and stress conditions was carried out. The total RNA sequencing generated a dataset of four samples, representing more than 40 Gb fastq raw data. A total of 145 million filtered reads were generated after the quality check. High-quality clean reads mapped rice reference genome RGAP 7. These aligned reads were taken for further analysis. A total of 11,867 DEGs across the genotypes under stress were identified. A total of 7577 and 4290 genes showed significant differential expression in BRC vs. BRIN library and HRC vs. HRIN library, respectively. The results reveal that differential gene expression analysis clearly shows the changes in the expression profiling of BR2655 and HR12 cultivars.

Item Type:	Article
Uncontrolled Keywords:	Transcriptomic analysis · Blast disease · Resistance genes · Rice plant · Differential gene expression · Fungal infection
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 04 Fungi 600 Technology > 08 Food technology > 21 Cereals > 01 Rice
Divisions:	Plant Cell Biotechnology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	08 Dec 2023 10:55
Last Modified:	08 Dec 2023 10:55
URI:	http://ir.cftri.res.in/id/eprint/16851

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