Optimization of a new heteropolysaccharide production by a native isolate of Leuconostoc sp. CFR-2181.

Vijayendra, S. V. N. and Babu, R S Sharath (2008) Optimization of a new heteropolysaccharide production by a native isolate of Leuconostoc sp. CFR-2181. Letters in applied microbiology, 46 (6). pp. 643-8. ISSN 1472-765X

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Abstract

AIM: This work is aimed at optimizing the production of a new heteropolysaccharide (HePS) of Leuconostoc sp. CFR-2181 by standardizing the fermentation conditions in a low cost semi-synthetic medium. METHODS AND RESULTS: Both nutritional and cultural parameters, such as carbon source and its concentration, initial pH of the exopolysaccharide (EPS) medium, fermentation temperature and fermentation period were optimized. Fermentation of the EPS medium (pH 6.7), containing sucrose at 5% (w/v) and 5% (v/v) inoculum, at 25 degrees C resulted in maximum production of HePS (18.38 g l(-1)) by the isolate in 4 h of fermentation. CONCLUSIONS: The isolate was found to produce good amount of HePS in just 4 h in a low cost semi-synthetic EPS medium. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first report on rapid production of HePS by any lactic culture, which can significantly reduce the cost of the EPS.

Item Type:	Article
Uncontrolled Keywords:	fermentation, heteropolysaccharide, Leuconostoc, optimization, semi-synthetic medium.
Subjects:	600 Technology > 05 Chemical engineering > 04 Fermentation Technology 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 02 Bacteriology
Divisions:	Food Microbiology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	12 Aug 2008 10:11
Last Modified:	28 Dec 2011 09:34
URI:	http://ir.cftri.res.in/id/eprint/1797

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