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A method for isolation of 2S, 7S, and 11S proteins of soyabean.
Appu Rao, A. G. and Narasinga Rao, M. S. (1977) A method for isolation of 2S, 7S, and 11S proteins of soyabean. Preparative Biochemistry, 7 (2). pp. 89-101.
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Abstract
A method is described for the isolation of 2S, 7S and 11S proteins of soybean. The unfractionated proteins are first precipitated with Hg(II) which yields a 11S-rich precipitate and this is further purified by (NH4)2SO4precipitation. From Mg(II)supernatant which is rich in 7S and 2S proteins, they are separated by the use of (NH4)2SO4 and cold ethanol. The 7S and 11S protein are obtained in a highly purified form as indicated by ultracentrifugation and polyacrylamide gel electrophoresis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | soybean, proteins |
| Subjects: | 600 Technology > 08 Food technology > 16 Nutritive value > 03 Proteins 600 Technology > 08 Food technology > 22 Legumes-Pulses > 05 Soya bean |
| Divisions: | Protein Chemistry and Technology |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 20 Mar 2018 04:17 |
| Last Modified: | 20 Mar 2018 04:17 |
| URI: | http://ir.cftri.res.in/id/eprint/2476 |
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