Suresh Chandra, B. R. and Appu Rao, A. G. and Narasinga Rao, M. S. (1986) Denaturation of glycinin by urea and guanidine hydrochloride. International Journal of Peptide and Protein Research, 27. pp. 493-500.
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Abstract
Denaturation of glycinin by urea and guanidine hydrochloride (GdnHC1) has
been studied at 30' by viscosity and circular dichroism (CD) measurements.
The kinetics of denaturation by 8M urea at 20, 30, 40 and SOo, and by 6M
GdnHCl at 20' have been measured by monitoring the increase in absorbance
at 287nm as a function of time. Viscosity increased with denaturant concentration
and reached maximum value of - 28 mL/g at 6M GdnHCl and 7M
urea, The (negative) molar ellipticity values in the region 250-200 nm decreased
with increase in denaturant concentration. Analysis of viscosity and CD data
indicated that both sets of data fitted the same curve of fd (fraction of protein
denatured) versus denaturant concentration. The kinetic data followed first
order reaction kinetics. These suggest that denaturation of glycinin by urea/
GdnHCl is a two-state process and follows the same pattern as that of globular
proteins.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | denaturation; equilibrium study; glycinin; guanidine hydrochloride; kinetic study; urea |
| Subjects: | 600 Technology > 08 Food technology > 16 Nutritive value > 03 Proteins 600 Technology > 08 Food technology > 22 Legumes-Pulses > 05 Soya bean |
| Divisions: | Protein Chemistry and Technology |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 14 Mar 2018 06:10 |
| Last Modified: | 14 Mar 2018 06:10 |
| URI: | http://ir.cftri.res.in/id/eprint/3693 |
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