Cloning of pediocin PA-1 and its immunity genes from Pediococcus acidilactici K7 using pAMJ shuttle vector into Lactococcus lactis MG1363

Srikanth, Anusha and Prakash, M. Halami (2008) Cloning of pediocin PA-1 and its immunity genes from Pediococcus acidilactici K7 using pAMJ shuttle vector into Lactococcus lactis MG1363. Indian Journal of Biotechnology, 7. pp. 550-553.

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Abstract

The matured pediocin PA-1 encoding gene, pedA and its immunity counterpart, pedB from Pediococcus acidilactici K7 were cloned by PCR technique and ligated into the E. coli-lactic shuttle, protein expression vector pAMJ2OO8. The recombinant pAML45 was constructed and sequenced to confirm the intactness of reading frame of the deduced pediocin PA-1 fusion protein. Lactococcus lactis MG1363 was electroporated with the plasmids and the transformation efficiency of ~ 2-3 × 103 μg-1 was observed. This was further confirmed by plasmid DNA isolation and analysis.

Item Type:	Article
Uncontrolled Keywords:	Pediococcus acidilactici, pediocin PA-1, electroporation, PI70 expression system, Lactococcus lactis
Subjects:	600 Technology > 08 Food technology > 16 Nutritive value > 03 Proteins 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 02 Bacteriology
Divisions:	Food Microbiology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	05 Jan 2009 05:10
Last Modified:	21 Dec 2016 12:49
URI:	http://ir.cftri.res.in/id/eprint/8867

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