Whey protein isolate enrichment attenuates manganese-induced oxidative stress and neurotoxicity in Drosophila melanogaster: Relevance to Parkinson’s disease.

Currently, much emphasis is being laid on understanding the role of nutrition to alleviate neurodegenerative
conditions. Here we employed a Drosophila model to test the hypothesis that Whey protein isolate (WPI) enrichment
can attenuate Manganese (Mn) induced locomotor phenotype, oxidative stress and neurotoxicity. Mn
exposure (0–30 mM) to young (8–10 d old) males/females and aged (30–35 d old) males elicited a concentration-
dependent temporal mortality and locomotor deficits. In general, females and aged males were more susceptible
than young males. Mn exposure (5–15 mM, 5 d) among male flies, besides induction of oxidative stress
(elevated levels of MDA and protein carbonyls with a concomitant decrease in glutathione levels), caused significant
perturbations in the activities of antioxidant enzymes and mitochondrial complexes. In the co-exposure
paradigm, WPI fortification (0.25–0.5%) markedly ameliorated Mn-induced locomotor phenotype, mortality,
oxidative stress and restored the activity of the antioxidant enzymes, cholinergic function, and dopamine levels.
In a satellite study, flies given prophylactic treatment (10 d) with WPI were more resistant(evidenced by a lower
incidence of mortality and locomotor deficits) to an acute MPP+ challenge suggesting its potential to enhance
survival pathways. Collectively, these data suggest the potential of WPI enrichment to attenuate Mn-induced
toxic manifestations in the Drosophila model. Based on these findings, we propose that fortification of diet with
WPI offers a useful approach to alleviate Mn-induced toxic manifestations and neuronal dysfunctions in humans,
especially in occupational settings.