Rapid semiquantitative estimation of benzene hexachloride by micro-t.l.c.

Visweswariah, K. and Jayaram, M. (1973) Rapid semiquantitative estimation of benzene hexachloride by micro-t.l.c. Pesticide Science, 4 (3). 283-287, 10 ref..

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Abstract

The relationship between spot concn. and the number of components separated during micro-TLC of BHC was investigated. Graded concn. (0.025 to greater than 1.5 mug) of BHC were spotted onto micro-TLC plates, developed with n-hexane and sprayed with chromogenic reagent o-tolidine. 2 components (alpha- and gamma-BHC) were observed with a spot concn. of 0.025 mug BHC; 5 components (heptachlorocyclohexane and alpha-, beta-, gamma- and delta-BHC) were observed at spot concn. greater than0.25% BHC. Comparative trials with macro-TLC (BHC concn. 1 to greater than 40 mug) showed micro-TLC to be 40 times more sensitive. For semiquantitative determination of BHC, it is desirable to dilute the insecticide extract to yield 2 spots with either micro- or macro-TLC as greater than 2 spots represents a wide range in spot concn. and increases the error in determination of the insecticide. The technique may be used for determination of BHC residues in grain or other foods.

Item Type:	Article
Uncontrolled Keywords:	BHC-; micro-TLC of BHC
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 26 Pesticide Chemistry 600 Technology > 08 Food technology > 01 Analysis
Divisions:	Food Protectants and Infestation Control
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	22 Dec 2016 06:22
Last Modified:	22 Dec 2016 06:22
URI:	http://ir.cftri.res.in/id/eprint/7191

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